Proteintech, 86693-2-RR, NQO1 Recombinant monoclonal antibody

Size: 20ul / 100ul The NQO1 (86693-2-RR) by Proteintech is a Recombinant antibody targeting NQO1 in WB, ELISA applications with reactivity to human, mouse, rat samples 86693-2-RR targets NQO1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, HSC-T6 cells, K-562 cells, HepG2 cells, NIH/3T3 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information NQO1, also named as DIA4, NMOR1, DTD and QR1, belongs to the NAD(P)H dehydrogenase (quinone) family. This enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. It is known to be involved in benzene metabolism. In human studies of ozone exposure, polymorphisms in oxidative stress genes (NQO1, GSTM1, GSTP1) modify respiratory symptoms, lung function, biomarkers and risk of asthma. (PMID:18511640, PMID:18848868) Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag2009 Product name: Recombinant human NQO1 protein Source: e coli. -derived, PKG Tag: GST Domain: 1-274 aa of BC007659 Sequence: MVGRRALIVLAHSERTSFNYAMKEAAAAALKKKGWEVVESDLYAMNFNPIISRKDITGKLKDPANFQYPAESVLAYKEGHLSPDIVAEQKKLEAADLVIFQFPLQWFGVPAILKGWFERVFIGEFAYTYAAMYDKGPFRSKKAVLSITTGGSGSMYSLQGIHGDMNVILWPIQSGILHFCGFQVLEPQLTYSIGHTPADARIQILEGWKKRLENIWDETPLYFAPSSLFDLNFQAGFLMKKEVQDEEKNKKFGLSVGHHLGKSIPTDNQIKARK Predict reactive species Full Name: NAD(P)H dehydrogenase, quinone 1 Calculated Molecular Weight: 274 aa, 31 kDa Observed Molecular Weight: 31 kDa GenBank Accession Number: BC007659 Gene Symbol: NQO1 Gene ID (NCBI): 1728 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P15559 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.