Proteintech, 86735-2-RR, TROAP Recombinant monoclonal antibody

Size: 20ul / 100ul The TROAP (86735-2-RR) by Proteintech is a Recombinant antibody targeting TROAP in WB, ELISA applications with reactivity to human samples 86735-2-RR targets TROAP in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HCT 116 cells, HeLa cells, PANC-1 cells, SK-BR-3 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Background Information TROAP, also known as tastin, was first cloned from human epithelial cells in 1995. It as a cytoplasmic protein is composed of 778 amino acid residues and contains potential phosphorylation sites for protein kinases. TROAP highly expresses in bone marrow, testis and thymus, and is involved in centrosome integrity and spindle assembly during mitosis (PMID: 33500384). TROAP has been shown to promote some tumor progressions, such as those of breast and colorectal cancers (PMID: 33692939). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag4562 Product name: Recombinant human TROAP protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 428-778 aa of BC032583 Sequence: QEVKIQRIGILQQLLRQEVEGLVGGQCVPLNGGSSLDMVELQPLLTEISRTLNATEHNSGTSHLPGLLKHSGLPKPCLPEECGEPQPCPPAEPGPPEAFCRSEPEIPEPSLQEQLEVPEPYPPAEPRPLESCCRSEPEIPESSRQEQLEVPEPCPPAEPRPLESYCRIEPEIPESSRQEQLEVPEPCPPAEPGPLQPSTQGQSGPPGPCPRVELGASEPCTLEHRSLESSLPPCCSQWAPATTSLIFSSQHPLCASPPICSLQSLRPPAGQAGLSNLAPRTLALRERLKSCLTAIHCFHEARLDDECAFYTSRAPPSGPTRVCTNPVATLLEWQDALCFIPVGSAAPQGSP Predict reactive species Full Name: trophinin associated protein (tastin) Calculated Molecular Weight: 778 aa, 84 kDa Observed Molecular Weight: 120 kDa GenBank Accession Number: BC032583 Gene Symbol: TROAP Gene ID (NCBI): 10024 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q12815 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.