Proteintech, 86938-1-RR, COX5A Recombinant monoclonal antibody

Size: 20ul / 100ul The COX5A (86938-1-RR) by Proteintech is a Recombinant antibody targeting COX5A in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 86938-1-RR targets COX5A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, mouse heart tissue, HepG2 cells, rat heart tissue, mouse brain tissue, rat brain tissue Positive IHC detected in: mouse heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000 Background Information COX5A(Cytochrome c oxidase subunit 5A, mitochondrial)is also named COXVA and belongs to the cytochrome c oxidase subunit 5A family. This is the heme A-containing chain of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. The mature human COX Va polypeptide shares 35% and 95% protein sequence identity with the corresponding S. cerevisiae COX subunit VI and bovine COX Va, respectively(PMID:2853101). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag2005 Product name: Recombinant human COX5A protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-150 aa of BC024240 Sequence: MLGAALRRCAVAATTRADPRGLLHSARTPGPAVAIQSVRCYSHGSQETDEEFDARWVTYFNKPDIDAWELRKGINTLVTYDMVPEPKIIDAALRACRRLNDFASTVRILEVVKDKAGPHKEIYPYVIQELRPTLNELGISTPEELGLDKV Predict reactive species Full Name: cytochrome c oxidase subunit Va Calculated Molecular Weight: 150 aa, 17 kDa Observed Molecular Weight: 13 kDa GenBank Accession Number: BC024240 Gene Symbol: COX5A Gene ID (NCBI): 9377 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P20674 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.