Proteintech, 86974-2-RR, HP1BP3 Recombinant monoclonal antibody

Size: 20ul / 100ul The HP1BP3 (86974-2-RR) by Proteintech is a Recombinant antibody targeting HP1BP3 in WB, ELISA applications with reactivity to human samples 86974-2-RR targets HP1BP3 in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells, MCF-7 cells, EC109 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information HP1BP3 (heterochromatin protein 1-binding protein 3) is an evolutionarily conserved nuclear protein that functions as a non-canonical linker histone. It is broadly expressed in most vertebrate tissues and is structurally related to the histone H1 family, containing three globular domains and a highly basic C-terminal tail reminiscent of H1 proteins . Unique to HP1BP3 are an acidic N-terminal stretch and a consensus HP1-binding motif (PxVxL), through which it associates with heterochromatin protein 1 (HP1α/β) and is enriched at perinucleolar heterochromatin regions marked by H3K9me3. Functionally, HP1BP3 acts as a modulator of gene expression and genome stability. Alternative splicing was reported to produce 4 different isoforms of HP1BP3, the calculated MW corresponding to 61, 57, 44, 15 kDa. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag18709 Product name: Recombinant human HP1BP3 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-302 aa of BC045660 Sequence: MASSPRPKMDAILTEAIKACFQKSGASVVAIRKYIIHKYPSLELERRGYLLKQALKRELNRGVIKQVKGKGASGSFVVVQKSRKTPQKSRNRKNRSSAVDPEPQVKLEDVLPLAFTRLCEPKEASYSLIRKYVSQYYPKLRVDIRPQLLKNALQRAVERGQLEQITGKGASGTFQLKKSGEKPLLGGSLMEYAILSAIAAMNEPKTCSTTALKKYVLENHPGTNSNYQMHLLKKTLQKCEKNGWMEQISGKGFSGTFQLCFPYYPSPGVLFPKKEPDDSRDEDEDEDESSEEDSEDEEPPPK Predict reactive species Full Name: heterochromatin protein 1, binding protein 3 Calculated Molecular Weight: 553 aa, 61 kDa Observed Molecular Weight: 75 kDa GenBank Accession Number: BC045660 Gene Symbol: HP1BP3 Gene ID (NCBI): 50809 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q5SSJ5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.