Proteintech, 87022-1-RR, CTNNA3 Recombinant monoclonal antibody

Size: 20ul / 100ul The CTNNA3 (87022-1-RR) by Proteintech is a Recombinant antibody targeting CTNNA3 in WB, IHC, IF-P, ELISA applications with reactivity to human, mouse, rat, canine samples 87022-1-RR targets CTNNA3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, canine samples. Tested Applications Positive WB detected in: HEK-293 cells, MDCK cells, C2C12 cells, C6 cells, PC-12 cells, mouse testis tissue, rat brain tissue Positive IHC detected in: rat heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: rat heart tissue, mouse heart tissue Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Background Information Alpha catenin is an essential component of adherens junctions that connects E-cadherin-β-catenin complexes with the actin cytoskeleton. The alpha catenin family of proteins consists of three members, alpha-E-catenin (CTNNA1), alpha-N-catenin (CTNNA2), and alpha-T-catenin (CTNNA3)(PMID: 21598020). CTNNA3 is a cell adhesion molecule. In intercalated discs of the heart, CTNNA3 is a component of a unique hybrid adhering junction, or area composita, that contains proteins associated with both desmosomes and adherens junctions (PMID: 22421363). Mutations in CTNNA3 are associated with arrhythmogenic right ventricular dysplasia, familial 13(PMID: 23136403). Specification Tested Reactivity: human, mouse, rat, canine Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag5008 Product name: Recombinant human CTNNA3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-376 aa of BC030646 Sequence: MSAETPITLNIDPQDLQVQTFTVEKLLEPLIIQVTTLVNCPQNPSSRKKGRSKRASVLLASVEEATWNLLDKGEKIAQEATVLKDELTASLEKVRKESEALKVSAERFTDDPCFLPKREAVVQAARALLAAVTRLLILADMIDVMCLLQHVSAFQRTFESLKNVANKSDLQKTYQKLGKELENLDYLAFKRQQDLKSPNQRDEIAGARASLKENSPLLHSICSACLEHSDVASLKASKDTVCEEIQNALNVISNASQGIQNMTTPPEPQAATLGSALDELENLIVLNPLTVTEEEIRPSLEKRLEAIISGAALLADSSCTRDLHRERIIAECNAIRQALQDLLSEYMNNLCNQKSDLGLTGVKIKLLEECIIFWSL Predict reactive species Full Name: catenin (cadherin-associated protein), alpha 3 Calculated Molecular Weight: 100 kDa Observed Molecular Weight: 100 kDa GenBank Accession Number: BC030646 Gene Symbol: CTNNA3 Gene ID (NCBI): 29119 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9UI47 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.