Proteintech, 87073-1-RR, Cadherin-13 Recombinant monoclonal antibody

Size: 20ul / 100ul The Cadherin-13 (87073-1-RR) by Proteintech is a Recombinant antibody targeting Cadherin-13 in WB, ELISA applications with reactivity to human, mouse, rat samples 87073-1-RR targets Cadherin-13 in WB, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: H9C2 cells, mouse stomach tissue, mouse heart tissue, rat skeletal muscle tissue, fetal human brain tissue, human heart tissue Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Cadherin-13 (CDH13, also known as H-cadherin or T-cadherin) is an atypical member of cadherin family, lacking transmembrane and cytosolic domains and anchored to the exterior surface of the plasma membrane via a GPI anchor. CDH13 is thought to affect cellular behavior largely through its signaling properties. It is highly expressed in heart, and in the CNS, CDH13 is expressed in cerebral cortex, medulla, hippocampus, amygdala, thalamus and substantia nigra. It is often down-regulated in cancerous cells. The CDH13 cDNA encodes a 713-amino acid protein, which has a calculated molecular weight of 78 kDa. Studies have detected variable bands on Western blots, including 105 kDa (mature form), 130 kDa (precursor), and 45 kDa, which may be a post-translational processing variant of CDH13 (PMID: 19399994). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Eg5964 Product name: Recombinant human CAD13 protein Source: mammalian cells -derived, V37 Tag: C-rFc Domain: 139-693 aa of NM_001257.4 Sequence: SIVVSPILIPENQRQPFPRDVGKVVDSDRPERSKFRLTGKGVDQEPKGIFRINENTGSVSVTRTLDREVIAVYQLFVETTDVNGKTLEGPVPLEVIVIDQNDNRPIFREGPYIGHVMEGSPTGTTVMRMTAFDADDPATDNALLRYNIRQQTPDKPSPNMFYIDPEKGDIVTVVSPALLDRETLENPKYELIIEAQDMAGLDVGLTGTATATIMIDDKNDHSPKFTKKEFQATVEEGAVGVIVNLTVEDKDDPTTGAWRAAYTIINGNPGQSFEIHTNPQTNEGMLSVVKPLDYEISAFHTLLIKVENEDPLVPDVSYGPSSTATVHITVLDVNEGPVFYPDPMMVTRQEDLSVGSVLLTVNATDPDSLQHQTIRYSVYKDPAGWLNINPINGTVDTTAVLDRESPFVDNSVYTALFLAIDSGNPPATGTGTLLITLEDVNDNAPFIYPTVAEVCDDAKNLSVVILGASDKDLHPNTDPFKFEIHKQAVPDKVWKISKINNTHALVSLLQNLNKANYNLPIMVTDSGKPPMTNITDLRVQVCSCRNSKVDCNAAG Predict reactive species Full Name: cadherin 13, H-cadherin (heart) Calculated Molecular Weight: 78 kDa Observed Molecular Weight: 105 kDa, 130 kDa GenBank Accession Number: NM_001257.4 Gene Symbol: Cadherin-13 Gene ID (NCBI): 1012 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P55290-1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.