Proteintech, 87078-1-RR, MDGA1 Recombinant monoclonal antibody

Size: 20ul / 100ul The MDGA1 (87078-1-RR) by Proteintech is a Recombinant antibody targeting MDGA1 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 87078-1-RR targets MDGA1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, rat brain tissue, fetal human brain tissue Positive IHC detected in: mouse cerebellum tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information The MAM domain GPI-anchored molecule (MDGA) family proteins are glycosylphosphatidylinositol (GPI) anchored proteins, consisting of MDGA1 and MDGA2. They are highly expressed in the central nervous system and can act as type-specific synaptic repressors (PMID: 41124857; 38900791). MDGA1 contains Ig and MAM domains that are involved in cell motility and play a role in cell migration and/or adhesion (PMID: 35532105). MDGA1 is a glycoprotein containing 955 amino acids with an observed molecular weight of 130-140 kDa (PMID: 35532105; 26776515; 21505559). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Eg5837 Product name: Recombinant human MDGA1 protein Source: mammalian cells -derived, V37 Tag: C-rFc Domain: 19-931 aa of NM_153487.4 Sequence: QGVYAPAQAQIVHAGQACVVKEDNISERVYTIREGDTLMLQCLVTGHPRPQVRWTKTAGSASDKFQETSVFNETLRIERIARTQGGRYYCKAENGVGVPAIKSIRVDVQYLDEPMLTVHQTVSDVRGNFYQEKTVFLRCTVNSNPPARFIWKRGSDTLSHSQDNGVDIYEPLYTQGETKVLKLKNLRPQDYASYTCQVSVRNVCGIPDKAITFRLTNTTAPPALKLSVNETLVVNPGENVTVQCLLTGGDPLPQLQWSHGPGPLPLGALAQGGTLSIPSVQARDSGYYNCTATNNVGNPAKKTVNLLVRSMKNATFQITPDVIKESENIQLGQDLKLSCHVDAVPQEKVTYQWFKNGKPARMSKRLLVTRNDPELPAVTSSLELIDLHFSDYGTYLCMASFPGAPVPDLSVEVNISSETVPPTISVPKGRAVVTVREGSPAELQCEVRGKPRPPVLWSRVDKEAALLPSGLPLEETPDGKLRLERVSRDMSGTYRCQTARYNGFNVRPREAQVQLNVQFPPEVEPSSQDVRQALGRPVLLRCSLLRGSPQRIASAVWRFKGQLLPPPPVVPAAAEAPDHAELRLDAVTRDSSGSYECSVSNDVGSAACLFQVSAKAYSPEFYFDTPNPTRSHKLSKNYSYVLQWTQREPDAVDPVLNYRLSIRQLNQHNAVVKAIPVRRVEKGQLLEYILTDLRVPHSYEVRLTPYTTFGAGDMASRIIHYTEPINSPNLSDNTCHFEDEKICGYTQDLTDNFDWTRQNALTQNPKRSPNTGPPTDISGTPEGYYMFIETSRPRELGDRARLVSPLYNASAKFYCVSFFYHMYGKHIGSLNLLVRSRNKGALDTHAWSLSGNKGNVWQQAHVPISPSGPFQIIFEGVRGPGYLGDIAIDDVTLKKGECPRKQTDPNKVVVMPG Predict reactive species Full Name: MAM domain containing glycosylphosphatidylinositol anchor 1 Calculated Molecular Weight: 106 kDa Observed Molecular Weight: 130 kDa GenBank Accession Number: NM_153487.4 Gene Symbol: MDGA1 Gene ID (NCBI): 266727 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q8NFP4-1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.