Proteintech, 87088-1-RR, Phospho-MYPT1 (Thr853) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-MYPT1 (Thr853) (87088-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-MYPT1 (Thr853) in WB, ELISA applications with reactivity to human, rat samples 87088-1-RR targets Phospho-MYPT1 (Thr853) in WB, ELISA applications and shows reactivity with human, rat samples. Tested Applications Positive WB detected in: HeLa cells, HEK-293 cells, C6 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Background Information Myosin phosphatase target subunit 1(MYPT1), which is also called PPP1R12A, is one of the subunits of myosin phosphatase. Phospho-MYPT1 (Thr853) is a phosphorylated form of MYPT1 (myosin phosphatase target subunit 1) at threonine 853. This phosphorylation event is associated with the regulation of smooth muscle contraction and cell motility. The Rho-associated protein kinase (ROCK) is known to phosphorylate MYPT1 at Thr853, which in turn suppresses the activity of myosin light chain phosphatase (MLCP), leading to an increase in myosin light chain phosphorylation and subsequent smooth muscle contraction. (PMID: 24712327) Specification Tested Reactivity: human, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: protein phosphatase 1, regulatory (inhibitor) subunit 12A Calculated Molecular Weight: 1030 aa, 115 kDa Observed Molecular Weight: 140 kDa GenBank Accession Number: BC111752 Gene Symbol: MYPT1 Gene ID (NCBI): 4659 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O14974 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.