Proteintech, 87127-3-RR, GNB1 Recombinant monoclonal antibody

Size: 20ul / 100ul The GNB1 (87127-3-RR) by Proteintech is a Recombinant antibody targeting GNB1 in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 87127-3-RR targets GNB1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells, Jurkat cells, T-47D cells, mouse brain tissue, rat brain tissue Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000 Background Information GNB1(Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1) belongs to the WD repeat G protein beta family.The guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems and the beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.GNB1 can form dimers with all gamma subunits analyzed(PMID:12782285). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag0313 Product name: Recombinant human GNB1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 2-339 aa of BC004186 Sequence: SELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIW Predict reactive species Full Name: guanine nucleotide binding protein (G protein), beta polypeptide 1 Calculated Molecular Weight: 37 kDa Observed Molecular Weight: 35 kDa GenBank Accession Number: BC004186 Gene Symbol: GNB1 Gene ID (NCBI): 2782 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P62873 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.