Proteintech, 87149-1-RR, Phospho-Vimentin (Ser56) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-Vimentin (Ser56) (87149-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-Vimentin (Ser56) in WB, ELISA applications with reactivity to human samples 87149-1-RR targets Phospho-Vimentin (Ser56) in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: paclitaxel treated Jurkat cells, paclitaxel treated HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information Vimentin, also named as VIM, belongs to the intermediate filament family. Phospho-Vimentin (Ser56) is a phosphorylated form of the intermediate filament protein Vimentin that plays a significant role in regulating cellular functions such as smooth muscle contraction, cell migration, and mitosis. Phosphorylation of Vimentin at Ser-56 has been found to regulate vimentin depolymerization and the spatial reorientation of vimentin filaments, which modulates intercellular force transmission and contraction in smooth muscle. (PMID: 27457922) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: vimentin Calculated Molecular Weight: 54 kDa Observed Molecular Weight: 57 kDa GenBank Accession Number: NM_003380 Gene Symbol: VIM Gene ID (NCBI): 7431 ENSEMBL Gene ID: ENSG00000026025 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P08670 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.