Proteintech, 87220-1-RR, Phospho-AKT (Thr450) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-AKT (Thr450) (87220-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-AKT (Thr450) in WB, ELISA applications with reactivity to human samples 87220-1-RR targets Phospho-AKT (Thr450) in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: MCF-7 cells, λ phosphatase treated MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Background Information The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. Phospho-AKT (Thr450) is recognized as the phosphorylation site on AKT (protein kinase B) at the threonine 450 residue. This site is referred to as the turn phosphorylation site and was previously thought to be a result of autophosphorylation, but more recent work has shown that its phosphorylation is controlled by TORC2 activity. This phosphorylation site is important in other AGC kinases, but the role in AKT signaling had not been determined. (PMID: 21670491) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: v-akt murine thymoma viral oncogene homolog 1 Calculated Molecular Weight: 56 kDa Observed Molecular Weight: 60 kDa GenBank Accession Number: NM_005163 Gene Symbol: AKT1 Gene ID (NCBI): 207 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P31749 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.