Proteintech, 87250-3-RR, PPM1G Recombinant monoclonal antibody

Size: 20ul / 100ul The PPM1G (87250-3-RR) by Proteintech is a Recombinant antibody targeting PPM1G in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 87250-3-RR targets PPM1G in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HCT 116 cells, HeLa cells, HEK-293T cells, Jurkat cells, MCF-7 cells Positive IHC detected in: mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000 Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag7751 Product name: Recombinant human PPM1G protein Source: e coli. -derived, T-HIS Tag: 6*His Domain: 191-546 aa of BC000057 Sequence: GPEDSTRETPSQENGPTAKAYTGFSSNSERGTEAGQVGEPGIPTGEAGPSCSSASDKLPRVAKSKFFEDSEDESDEAEEEEEDSEECSEEEDGYSSEEAENEEDEDDTEEAEEDDEEEEEEMMVPGMEGKEEPGSDSGTTAVVALIRGKQLIVANAGDSRCVVSEAGKALDMSYDHKPEDEVELARIKNAGGKVTMDGRVNGGLNLSRAIGDHFYKRNKNLPPEEQMISALPDIKVLTLTDDHEFMVIACDGIWNVMSSQEVVDFIQSKISQRDENGELRLLSSIVEELLDQCLAPDTSGDGTGCDNMTCIIICFKPRNTAELQPESGKRKLEEVLSTEGAEENGNSDKKKKAKRD Predict reactive species Full Name: protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform Calculated Molecular Weight: 59 kDa Observed Molecular Weight: 70 kDa GenBank Accession Number: BC000057 Gene Symbol: PPM1G Gene ID (NCBI): 5496 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O15355 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.