Proteintech, 87289-1-RR, Phospho-LAT (Tyr220) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-LAT (Tyr220) (87289-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-LAT (Tyr220) in WB, ELISA applications with reactivity to human samples 87289-1-RR targets Phospho-LAT (Tyr220) in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Jurkat cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Background Information LAT is a membrane-localized adaptor protein which is primarily concentrated in microdomains by palmitoylation. Phospho-LAT (Tyr220) is a phosphorylated form of the Linker for Activation of T cells (LAT), a protein that plays a pivotal role in T cell receptor (TCR) signaling. LAT is involved in the assembly of the signaling complex that leads to T cell activation. Upon TCR engagement, LAT becomes phosphorylated at multiple tyrosine residues, including Tyr220. (PMID: 38746248) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: linker for activation of T cells Calculated Molecular Weight: 262 aa, 28 kDa Observed Molecular Weight: 36 kDa, 38 kDa GenBank Accession Number: BC011563 Gene Symbol: LAT Gene ID (NCBI): 27040 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O43561 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.