Proteintech, 87322-1-RR, Phospho-ALK (Tyr1507) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-ALK (Tyr1507) (87322-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-ALK (Tyr1507) in WB, ELISA applications with reactivity to human samples 87322-1-RR targets Phospho-ALK (Tyr1507) in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Karpas-299 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Background Information Anaplastic lymphoma kinase was first described in 1994 as the NPM-ALK fusion protein that is expressed in the majority of anaplastic large-cell lymphomas. About 2/3rds of anaplastic large-cell lymphomas possess a balanced chromosomal translocation in which the entire nucle-ophosmin (NPM) gene on chromosome 5 is fused to the 3' portion of the ALK gene (including the entire intracellular segment with its protein kinase domain) on chromosome 2. This oncogenic ALK protein kinase is a chimeric protein created by a translocation between chromosomes(2;5)(p23;q35) that generates the NPM-ALK fusion protein. NPM-ALK also known as p80, since the chimeric protein weighs 80 kDa. Studies of NPM-ALK have identified pTyr1096 as the binding site for insulin receptor substrate 1 (IRS1); pTyr1507 is the binding site for Shc and pTyr1604 is the binding site for PLC-Y (native human ALK receptor residue numbers are given; subtract 940 to obtain the NPM-ALK residue). (PMID: 23201355, PMID: 33466277) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: anaplastic lymphoma receptor tyrosine kinase Observed Molecular Weight: 80 kDa GenBank Accession Number: BC157090 Gene Symbol: ALK Gene ID (NCBI): 238 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9UM73 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.