Proteintech, 87327-1-RR, Phospho-BCR (Tyr177) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-BCR (Tyr177) (87327-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-BCR (Tyr177) in WB, ELISA applications with reactivity to human samples 87327-1-RR targets Phospho-BCR (Tyr177) in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: K-562 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Background Information The Bcr gene, mapping on chromosome22, was orginally identified by its presence in the chimeric Bcr-Abl oncogene. The aminoterminal BCR-encoded sequences of BCR-ABL contain a tyrosine-phosphorylated site that binds the SH2 domain of the adaptor protein GRB2. It is now evident that the phosphorylation of BCR Tyr177 is essential for BCR-ABL-mediated leukemogenesis, and its mutation largely abolishes GRB2 binding and diminishes BCR-ABL-induced Ras activation. (PMID: 22156549) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: breakpoint cluster region Calculated Molecular Weight: 143 kDa Observed Molecular Weight: 210 kDa GenBank Accession Number: NM_004327 Gene Symbol: BCR Gene ID (NCBI): 613 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P11274 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.