Proteintech, 87394-1-RR, Phospho-NPM1 (Thr199) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-NPM1 (Thr199) (87394-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-NPM1 (Thr199) in WB, ELISA applications with reactivity to human samples 87394-1-RR targets Phospho-NPM1 (Thr199) in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: paclitaxel treated HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information Nucleophosmin (NPM1,B23) is a putative ribosome assembly factor with a high affinity for peptides containing nuclear localization signals (NLSs). Phospho-NPM1 (Thr199) is a phosphorylated form of nucleophosmin (NPM1) at threonine 199, a residue that plays a critical role in cell cycle regulation and cancer development. Indeed, phosphorylation by CDK2/cyclin E on Thr199 residue during G1 phase is critical for the NPM1 dissociation from the centrosome to initiate its duplication and the pre-mRNA processing. (PMID: 26993766) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: nucleophosmin (nucleolar phosphoprotein B23, numatrin) Calculated Molecular Weight: 33 kDa Observed Molecular Weight: 40 kDa GenBank Accession Number: NM_002520 Gene Symbol: NPM1 Gene ID (NCBI): 4869 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P06748 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.