Proteintech, 87497-1-RR, CD300f Recombinant monoclonal antibody

Size: 20ul / 100ul The CD300f (87497-1-RR) by Proteintech is a Recombinant antibody targeting CD300f in WB, ELISA applications with reactivity to human samples 87497-1-RR targets CD300f in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: U-937 cells, HL-60 cells, THP-1 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Background Information CD300f (also known as CLM-1 or IREM-1) is a type I transmembrane glycoprotein belonging to the CD300 family of immunoreceptors. Expressed predominantly on myeloid cells, including macrophages, dendritic cells, and mast cells, CD300f functions as an inhibitory receptor that regulates immune responses. Its extracellular domain contains a single IgV-like domain that recognizes phosphatidylserine and phosphatidylethanolamine, enabling recognition of apoptotic cells. The cytoplasmic tail harbors immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that recruit phosphatases SHP-1 and SHP-2 upon phosphorylation, delivering negative signals. CD300f plays critical roles in dampening inflammation, promoting efferocytosis, and maintaining immune homeostasis (PMID: 28073916, PMID: 33548578, PMID: 37302321). The predicted molecular weight of CD300f is 32 kDa; however, due to different levels of N- and/or O-linked glycosylation modifications, its molecular weight may range from 40 to 60 kDa (PMID: 15549731, PMID: 31338922). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Eg10440 Product name: Recombinant human CD300F protein Source: mammalian cells -derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 10011473 of Sequence: \ Predict reactive species Full Name: CD300 molecule-like family member f Observed Molecular Weight: 40-60 kDa Gene Symbol: CD300LF Gene ID (NCBI): 146722 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q8TDQ1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.