Proteintech, 87619-1-RR, Phospho-MSK1 (Ser376) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-MSK1 (Ser376) (87619-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-MSK1 (Ser376) in WB, ELISA applications with reactivity to human samples 87619-1-RR targets Phospho-MSK1 (Ser376) in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Calyculin A treated HeLa cells, PMA treated HEK-293T cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Background Information Mitogen- and stress-activated kinase 1 (MSK1) is a protein kinase that plays a crucial role in the regulation of gene expression in response to various stimuli, including mitogens, stress, and inflammatory signals. ERK1/2 or p38 MAPK activate MSK1 by phosphorylating Thr581 and Ser360; this primes the C-terminal domain, which then autophosphorylates Ser376 (and Ser212) to unlock the N-terminal catalytic site. (PMID: 15568999) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: ribosomal protein S6 kinase, 90kDa, polypeptide 5 Calculated Molecular Weight: 549aa,62 kDa; 802aa,90 kDa Observed Molecular Weight: 90 kDa GenBank Accession Number: BC017187 Gene Symbol: MSK1 Gene ID (NCBI): 9252 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O75582 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.