Proteintech, 98052-1-RR, Anti-Mouse IL-1 alpha Rabbit Recombinant Antibody

Size: 100ug The IL-1 alpha (98052-1-RR) by Proteintech is a Recombinant antibody targeting IL-1 alpha in FC (Intra) applications with reactivity to mouse samples 98052-1-RR targets IL-1 alpha in FC (Intra) applications and shows reactivity with mouse samples. Tested Applications Positive FC (Intra) detected in: LPS treated RAW 264.7 cells Recommended dilution Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in 100 μl suspension Background Information IL-1 alpha is a dual function cytokine that can function both as a transcription factor and a damage-associated molecular pattern (DAMP) in innate immune responses. It is expressed by many cell types and its expression is increased when cells are exposed to growth factors, pro-inflammatory, and stress-related signals. In its pro-form, IL-1 alpha contains a nuclear localization signal that allows it to translocate to the nucleus and bind to DNA, resulting in either the increase or prohibition of pro-inflammatory cytokine expression. When cells are exposed to necrotic signals, mature IL-1 alpha is released from the cell lysates and triggers a strong pro-inflammatory response upon binding to its receptor on nearby cells. Due to its strongly pro-inflammatory nature, IL-1 alpha has been linked to tumor metastasis and lower median survival in several cancers (PMID: 27434011, 29247995). Specification Tested Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Fusion Protein Predict reactive species Full Name: interleukin 1 alpha Calculated Molecular Weight: 31kd GenBank Accession Number: NM_010554 Gene Symbol: Il1a Gene ID (NCBI): 16175 RRID: AB_3672199 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P01582 Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2-8°C. Stable for one year after shipment.