Proteintech, 98178-1-RR, Anti-Human IL-5 Rabbit Recombinant Antibody

Size: 100ug The IL-5 (98178-1-RR) by Proteintech is a Recombinant antibody targeting IL-5 in FC (Intra) applications with reactivity to human samples 98178-1-RR targets IL-5 in FC (Intra) applications and shows reactivity with human samples. Tested Applications Positive FC (Intra) detected in: PMA, Ionomycin and Brefeldin A treated human PBMCs Recommended dilution Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in 100 μl suspension Background Information IL-5 is a 4-α-helix, pleiotropic cytokine that regulates the activities of B cells, eosinophils, and basophils. It is produced mostly by CD4+ T cells, eosinophils, and basophils, and signals through its specific receptor (IL-5R) on target cells to activate downstream JAK/STAT, Btk, and Ras/ERk pathways. Due to its pleiotropic effects, IL-5 plays a role in both innate and adaptive immune responses. It is a strong promoter of eosinophil differentiation, migration, maturation, and survival. Additionally, IL-5 is also capable of stimulating activated B cells to differentiate into antibody-secreting cells. Elevated IL-5 levels are strongly correlated with eosinophilia, which is a hallmark feature of allergy, asthma, and hypersensitivity. (PMID: 21986312, 22174445, 19819937, 16895557) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Eg0380 Product name: Recombinant Human IL-5 protein (His Tag) Source: mammalian cells -derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 20-134 aa of BC066282 Sequence: IPTEIPTSALVKETLALLSTHRTLLIANETLRIPVPVHKNHQLCTEEIFQGIGTLESQTVQGGTVERLFKNLSLIKKYIDGQKKKCGEERRRVNQFLDYLQEFLGVMNTEWIIES Predict reactive species Full Name: interleukin 5 (colony-stimulating factor, eosinophil) Calculated Molecular Weight: 134 aa, 15 kDa GenBank Accession Number: BC066282 Gene Symbol: IL5 Gene ID (NCBI): 3567 RRID: AB_3672321 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P05113 Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.