Product Description
Size: 100ug
The Whitlow/218 Linker (98672-3-RR) by Proteintech is a Recombinant antibody targeting Whitlow/218 Linker in IHC, IF/ICC, FC applications with reactivity to n/a samples
98672-3-RR targets Whitlow/218 Linker in IHC, IF/ICC, FC applications and shows reactivity with n/a samples.
Tested Applications
Positive IHC detected in: 293T cell(218Linker)Note: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive IF/ICC detected in: Transfected CHO cells
Positive FC detected in: Transfected HEK-293T cells
Recommended dilution
Immunohistochemistry (IHC): IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension
Background Information
The 218 Linker (also known as the Whitlow linker) is a synthetic peptide sequence, connecting two protein domains, most commonly the Variable Heavy (VH) and Variable Light (VL) chains of an antibody, to form a stable single-chain variable fragment (scFv) (PMID: 8309948). Its standard amino acid sequence is GSTSGSGKPGSGEGSTKG.
Specification
Tested Reactivity: n/a
Host / Isotype: Rabbit / IgG
Class: Recombinant
Type: Antibody
Immunogen: Peptide Predict reactive species
Full Name: Whitlow/218 Linker
Conjugate: Unconjugated
Form: Liquid
Purification Method: Protein A purification
Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.
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Collaboration
Tony Tang
Email: Tony.Tang@iright.com
Mobile/WhatsApp/Wechat: +86-17717886924