Proteintech, APC-FcA98143-2, FcZero-rAb™ APC Anti-Human Ki-67 Rabbit Recombinant Antibody

Size: 100tests The Ki-67 (APC-FcA98143-2) by Proteintech is a Recombinant antibody targeting Ki-67 in FC (Intra) applications with reactivity to human samples APC-FcA98143-2 targets Ki-67 in FC (Intra) applications and shows reactivity with human samples. Tested Applications Positive FC (Intra) detected in: PHA treated human PBMCs Recommended dilution Flow Cytometry (FC) (INTRA): FC (INTRA) : 5 ul per 10^6 cells in a 100 µl suspension Background Information The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: antigen identified by monoclonal antibody Ki-67 Calculated Molecular Weight: 359 kDa GenBank Accession Number: NM_002417 Gene Symbol: KI67 Gene ID (NCBI): 4288 Conjugate: APC Fluorescent Dye Excitation/Emission Maxima Wavelengths: 650 nm / 660 nm Form: Liquid Purification Method: Protein A purification UNIPROT ID: P46013 Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.