Proteintech, APC-FcA98177, FcZero-rAb™ APC Anti-Human Siglec-8 Rabbit Recombinant Antibody

Size: 100tests The Siglec-8 (APC-FcA98177) by Proteintech is a Recombinant antibody targeting Siglec-8 in FC applications with reactivity to human samples APC-FcA98177 targets Siglec-8 in FC applications and shows reactivity with human samples. Tested Applications Positive FC detected in: human peripheral blood leukocytes Recommended dilution Flow Cytometry (FC): FC : 5 ul per 10^6 cells in a 100 µl suspension Background Information Sialic acid-binding Ig-like lectin 8 (Siglec-8), also known as SAF2, is a sialic acid-binding immunoglobulin-like lectin that belongs to the SIGLEC family of the Ig superfamily (PMID: 10856141). Siglec-8 is a single-pass transmembrane protein containing three extracellular immunoglobulin-like domains, a transmembrane region, and a cytoplasmic tail (PMID: 10625619). It is an inhibitory receptor selectively expressed on human mast cells and eosinophils, and at low levels on basophils (PMID: 10856141; 33374255). Engagement of Siglec-8 with antibody results in inhibiting IgE-mediated mast cell responses and reducing allergic inflammation (PMID: 32814824). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Recombinant Protein Predict reactive species Full Name: sialic acid binding Ig-like lectin 8 Calculated Molecular Weight: 499 aa, 54 kDa GenBank Accession Number: BC053319 Gene Symbol: SIGLEC8 Gene ID (NCBI): 27181 Conjugate: APC Fluorescent Dye Excitation/Emission Maxima Wavelengths: 650 nm / 660 nm Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9NYZ4 Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.