Proteintech, APC-FcA98450, FcZero-rAb™ APC Anti-6*His, His-Tag Rabbit Recombinant Antibody

Size: 100ug The 6*His, His-Tag (APC-FcA98450) by Proteintech is a Recombinant antibody targeting 6*His, His-Tag in FC (Intra) applications with reactivity to n/a samples APC-FcA98450 targets 6*His, His-Tag in FC (Intra) applications and shows reactivity with n/a samples. Tested Applications Positive FC (Intra) detected in: Transfected HEK-293 cells Recommended dilution Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.1 ug per 10^6 cells in a 100 µl suspension Background Information Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His-tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His-tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells. Specification Tested Reactivity: n/a Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: unknow Predict reactive species Full Name: 6*His, His-Tag Calculated Molecular Weight: 1 kDa Conjugate: APC Fluorescent Dye Excitation/Emission Maxima Wavelengths: 650 nm / 660 nm Form: Liquid Purification Method: Protein A purification UNIPROT ID: HISTAG Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.