Proteintech, Biotin-FcA98262, FcZero-rAb™ Biotin Anti-GGGGS Linker Rabbit Recombinant Antibody

Size: 100ug The GGGGS Linker (Biotin-FcA98262) by Proteintech is a Recombinant antibody targeting GGGGS Linker in FC, ELISA applications with reactivity to recombinant protein samples Biotin-FcA98262 targets GGGGS Linker in FC, ELISA applications and shows reactivity with recombinant protein samples. Tested Applications Positive FC detected in: Transfected HEK-293T cells Recommended dilution Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information As a crucial element in the design of recombinant fusion proteins, linkers play an increasingly vital role in the construction of stable, bioactive fusion proteins (PMID: 23026637). GGGGS linker (G4S linker) is a flexible linker made of 4 glycine repeats followed by a serine amino acid (PMID:3045807). Due to its flexibility and resistance to proteases, GGGGS and its repeats are commonly used when engineering a protein, particularly in the construction of single-chain Fv (ScFv) domains expressed on the surfaces of CAR-T cells (PMID:23581628; 36874404). This antibody, raised against a synthetic peptide (GGGGSGGGGSGGGGS), can recognize G4S*2, G4S*3, G4S*4, and G4S*5. Specification Tested Reactivity: recombinant protein Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: GGGGS Linker Conjugate: Biotin Excitation/Emission Maxima Wavelengths: - Form: Liquid Purification Method: Protein A purification Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.