Proteintech, CL488-65106, CoraLite® Plus 488 Anti-Mouse TCR Beta (H57-597)

Size: 100ug The TCR Beta (CL488-65106) by Proteintech is a Monoclonal antibody targeting TCR Beta in IF/ICC, FC applications with reactivity to mouse samples CL488-65106 targets TCR Beta in IF/ICC, FC applications and shows reactivity with mouse samples. Tested Applications Positive IF/ICC detected in: mouse splenocytes Positive FC detected in: mouse splenocytes Recommended dilution Immunofluorescence (IF)/ICC: IF/ICC : 1:150-1:600 Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension Background Information T-cell receptor (TCR) consists of heterodimeric glycoproteins (TCR alpha/beta or gamma/delta) that demonstrate homology with immunoglobulins (PMID: 2144901). TCR beta is a member of the immunoglobulin superfamily and is expressed on alpha/beta TCR-bearing T cells and thymocytes. The TCR heterodimers are noncovalently associated with CD3. The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta (PMID: 1826255). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657). Specification Tested Reactivity: mouse Host / Isotype: Armenian Hamster / IgG Class: Monoclonal Type: Antibody Immunogen: Affinity purified TCR from mouse DO-11.10 cells Predict reactive species Full Name: T-cell receptor beta chain Gene Symbol: TCR beta Gene ID (NCBI): 21577 RRID: AB_2883180 Conjugate: CoraLite® Plus 488 Fluorescent Dye Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm Form: Liquid Purification Method: Affinity purification Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.