Proteintech, CL488-66902, CoraLite® Plus 488-conjugated AIM2 Monoclonal antibody

Size: 100ul The AIM2 (CL488-66902) by Proteintech is a Monoclonal antibody targeting AIM2 in WB, IF/ICC applications with reactivity to human, mouse, rat, rabbit samples CL488-66902 targets AIM2 in WB, IF/ICC applications and shows reactivity with human, mouse, rat, rabbit samples. Tested Applications Positive WB detected in: RAW 264.7 cells Positive IF/ICC detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Absent in melanoma 2 (AIM2) is a sensor of cytosolic DNA that is responsible for activation of the inflammasome and host immune responses to DNA viruses and intracellular bacteria [PMID: 22695634]. Upon binding to DNA, it is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Otherwise, it functions as a tumor suppressor which may act by repressing NF-kappa-B transcriptional activity [PMID:16432157,20566831]. 66902-1-Ig antibody detects the 37-39 and 53 kDa protein in SDS-PAGE similar to the paper published. [PMID: 28809949] Specification Tested Reactivity: human, mouse, rat, rabbit Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag14607 Product name: Recombinant human AIM2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-343 aa of BC010940 Sequence: MESKYKEILLLTGLDNITDEELDRFKFFLSDEFNIATGKLHTANRIQVATLMIQNAGAVSAVMKTIRIFQKLNYMLLAKRLQEEKEKVDKQYKSVTKPKPLSQAEMSPAASAAIRNDVAKQRAAPKVSPHVKPEQKQMVAQQESIREGFQKRCLPVMVLKAKKPFTFETQEGKQEMFHATVATEKEFFFVKVFNTLLKDKFIPKRIIIIARYYRHSGFLEVNSASRVLDAESDQKVNVPLNIIRKAGETPKINTLQTQPLGTIVNGLFVVQKVTEKKKNILFDLSDNTGKMEVLGVRNEDTMKCKEGDKVRLTFFTLSKNGEKLQLTSGVHSTIKVIKAKKKK Predict reactive species Full Name: absent in melanoma 2 Calculated Molecular Weight: 343 aa, 39 kDa Observed Molecular Weight: 37-39 and 53 kDa GenBank Accession Number: BC010940 Gene Symbol: AIM2 Gene ID (NCBI): 9447 RRID: AB_3084284 Conjugate: CoraLite® Plus 488 Fluorescent Dye Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm Form: Liquid Purification Method: Protein G purification UNIPROT ID: O14862 Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage.