Proteintech, CL488-FcA98167, FcZero-rAb™ CoraLite® Plus 488 Anti-Mouse LAG-3/CD223 Rabbit Recombinant Antibody

Size: 100ug The LAG-3/CD223 (CL488-FcA98167) by Proteintech is a Recombinant antibody targeting LAG-3/CD223 in FC applications with reactivity to mouse samples CL488-FcA98167 targets LAG-3/CD223 in FC applications and shows reactivity with mouse samples. Tested Applications Positive FC detected in: Anti-CD3/CD28 treated mouse splenocytes Recommended dilution Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Lymphocyte activation gene 3 protein (LAG-3), also known as CD223, is a type I transmembrane protein belonging to the immunoglobulin superfamily (PMID: 1692078). It is a CD4-related molecule that binds MHC class II with high affinity (PMID: 1692078; 9159144). LAG-3 is expressed on activated T and NK cells. Besides, natural CD4(+)CD25(+) Tregs express LAG-3 upon activation (PMID: 15485628). LAG-3 negatively regulates T-cell function and homeostasis, and contributes to the suppressor activity of Tregs (PMID: 14644131; 15485628). Specification Tested Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Eg1220 Product name: Recombinant Mouse LAG-3/CD223 protein (His Tag) Source: mammalian cells -derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 24-442 aa of NM_008479.2 Sequence: GPGKELPVVWAQEGAPVHLPCSLKSPNLDPNFLRRGGVIWQHQPDSGQPTPIPALDLHQGMPSPRQPAPGRYTVLSVAPGGLRSGRQPLHPHVQLEERGLQRGDFSLWLRPALRTDAGEYHATVRLPNRALSCSLRLRVGQASMIASPSGVLKLSDWVLLNCSFSRPDRPVSVHWFQGQNRVPVYNSPRHFLAETFLLLPQVSPLDSGTWGCVLTYRDGFNVSITYNLKVLGLEPVAPLTVYAAEGSRVELPCHLPPGVGTPSLLIAKWTPPGGGPELPVAGKSGNFTLHLEAVGLAQAGTYTCSIHLQGQQLNATVTLAVITVTPKSFGLPGSRGKLLCEVTPASGKERFVWRPLNNLSRSCPGPVLEIQEARLLAERWQCQLYEGQRLLGATVYAAESSSGAHSARRISGDLKGGHL Predict reactive species Full Name: lymphocyte-activation gene 3 Calculated Molecular Weight: 57 kDa GenBank Accession Number: NM_008479.2 Gene Symbol: CD223 Gene ID (NCBI): 16768 Conjugate: CoraLite® Plus 488 Fluorescent Dye Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q61790 Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.