Proteintech, CL555-pd1, ChromoTek anti-PD-1 recombinant VHH, CoraLite® Plus 555

Size: 150ul CL555-pd1 targets PD-1 in FC applications and shows reactivity with Human samples. Specification Applications: FC Reactivity: Human Conjugate: CoraLite® Plus 555 Type: Nanobody Class: Recombinant Host: Alpaca Affinity: Picomolar range, below the assay limit (biolayer interferometry) Molecular Weight: 13.874 kDa Form: Liquid Excitation/Emission Maxima Wavelengths: 554 nm / 570 nm RRID: AB_3101930 Storage Buffer: 500 mM NaCl, 10 mM HEPES pH 7.0, 5 mM EDTA, 0.09% sodium azide Storage Condition: Store at -20°C Background: Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436). It has been reported that PD-1 is heavily glycosylated and migrates with an apparent molecular mass of 47-55 kDa on SDS-PAGE, which is larger than its predicted mass of 32 kDa (PMID: 8671665; 17640856; 17003438).