Proteintech, CL555-tmt, ChromoTek anti-TIM3 recombinant VHH, CoraLite® Plus 555

Size: 150ul CL555-tmt targets TIM3 in FC and IF applications and shows reactivity with Human samples. Specification Applications: FC, IF Reactivity: Human Conjugate: CoraLite® Plus 555 Type: Nanobody Class: Recombinant Host: Alpaca Affinity: Picomolar range, below the assay limit (biolayer interferometry) Molecular Weight: 14.824 kDa Form: Liquid Excitation/Emission Maxima Wavelengths: 554 nm / 570 nm RRID: AB_3101921 Storage Buffer: 500 mM NaCl, 10 mM HEPES pH 7.0, 5 mM EDTA, 0.09% sodium azide Storage Condition: Store at -20°C; Shipped at ambient temperature. Background: TIM3, also known as HAVCR2, is a member of the recently discovered T cell Ig and mucin domain-containing molecule superfamily. TIM3 is a negative regulatory molecule that is important for T cell tolerance and has a crucial role in autoimmunity and T cell exhaustion during chronic viral infection (PMID: 23180819). TIM3 is expressed by T-helper type 1 (Th1) cells, macrophage, monocyte, dendritic cells, CD8+ T cell and other lymphocyte subsets. Galectin-9 is a ligand for TIM3. TIM3-galectin-9 pathway negatively regulates T helper type 1 immunity (PMID: 16286920). TIM3 is a 280-aa membrane protein with a calculated molecular weight of 33 kDa, the higher molecular weights between 50 and 70 kDa detected by this monoclonal antibody probably represent glycosylated TIM3 (PMID: 20107545; 17069754; 11725301).