Proteintech, CL594-65061, CoraLite®594 Anti-Mouse CD3e (500-A2)

Size: 100ug The CD3e (CL594-65061) by Proteintech is a Monoclonal antibody targeting CD3e in FC applications with reactivity to mouse samples CL594-65061 targets CD3e in FC applications and shows reactivity with mouse samples. Tested Applications Positive FC detected in: mouse splenocytes Recommended dilution Flow Cytometry (FC): FC : 0.5 ug per 10^6 cells in 100 μl suspension Background Information CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657). Specification Tested Reactivity: mouse Host / Isotype: Syrian Hamster / IgG Class: Monoclonal Type: Antibody Immunogen: Immune complexes isolated from C6VL-BS thymoma cells Predict reactive species Full Name: CD3 antigen, epsilon polypeptide GenBank Accession Number: BC098236 Gene Symbol: CD3ε Gene ID (NCBI): 12501 RRID: AB_2919930 Conjugate: CoraLite®594 Fluorescent Dye Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm Form: Liquid Purification Method: Affinity purification UNIPROT ID: P22646 Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.