Proteintech, CL647-65083, CoraLite® Plus 647 Anti-Human CD80 (2D10.4)

Size: 100tests The CD80 (CL647-65083) by Proteintech is a Monoclonal antibody targeting CD80 in FC applications with reactivity to human samples CL647-65083 targets CD80 in FC applications and shows reactivity with human samples. Tested Applications Positive FC detected in: Daudi cells, IFN-γ and LPS-stimulated THP-1-derived macrophages Recommended dilution Flow Cytometry (FC): FC : 5 ul per 10^6 cells in 100 μl suspension Background Information CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215) Specification Tested Reactivity: human Host / Isotype: Mouse / IgG1, kappa Class: Monoclonal Type: Antibody Immunogen: N/A Predict reactive species Full Name: CD80 molecule Calculated Molecular Weight: 33 kDa GenBank Accession Number: BC042665 Gene Symbol: CD80 Gene ID (NCBI): 941 RRID: AB_2883670 Conjugate: CoraLite® Plus 647 Fluorescent Dye Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm Form: Liquid Purification Method: Affinity purification UNIPROT ID: P33681 Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.