Proteintech, CL647-FcA98449, FcZero-rAb™ CoraLite® Plus 647 Anti-DYKDDDDK tag Rabbit Recombinant Antibody (Binds to FLAG® tag epitope)

Size: 100ug The DYKDDDDK tag (CL647-FcA98449) by Proteintech is a Recombinant antibody targeting DYKDDDDK tag in FC (Intra) applications with reactivity to n/a samples CL647-FcA98449 targets DYKDDDDK tag in FC (Intra) applications and shows reactivity with n/a samples. Tested Applications Positive FC (Intra) detected in: Transfected HEK-293T cells Recommended dilution Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DDDDK peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology. Specification Tested Reactivity: n/a Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: unknow Predict reactive species Full Name: DYKDDDDK tag Conjugate: CoraLite® Plus 647 Fluorescent Dye Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm Excitation Laser: Red Laser (633 nm) Form: Liquid Purification Method: Protein A purification UNIPROT ID: FLAGTAG Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.