Proteintech, SF65666-1-TR, Anti-Mouse CD8a Rat scFv Antibody

Size: 100ug The CD8a (SF65666-1-TR) by Proteintech is a Recombinant antibody targeting CD8a in FC applications with reactivity to mouse samples SF65666-1-TR targets CD8a in FC applications and shows reactivity with mouse samples. Tested Applications Positive FC detected in: mouse splenocytes Recommended dilution Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells, on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084). Specification Tested Reactivity: mouse Host / Type: Rat / scFv Class: Recombinant Type: Single-chain variable fragment antibody (scFv) Immunogen: N/A Predict reactive species Full Name: CD8 antigen, alpha chain GenBank Accession Number: BC030679 Gene Symbol: Cd8a Gene ID (NCBI): 12525 Conjugate: Unconjugated Source: Anti-Mouse CD8a scFv from clone 53-6.7, consisting of VH-GGGGS linker-VL (N- to C-terminus), with a His-C tag at the C-terminus. Form: Liquid Purification Method: Protein A purification Storage Buffer: 50mM HEPES, 150mM NaCl, 5mM EDTA, 0.1% CHAPS, 10% Sucrose and 0.1% Proclin 300, pH 7.3. Storage Conditions: Store at 2-8°C. Stable for one year after shipment. 20ul sizes contain 0.1% BSA.