Proteintech, shuGCL488-2, ChromoTek Nano-Secondary® anti-human IgG, recombinant VHH, CoraLite® Plus 488 [CTK0117]

Size: 10ul / 100ul Nano-Secondary® anti-human IgG, recombinant VHH, is a human IgG-specific (IgG1, IgG2, IgG3, IgG4) antibody. This secondary antibody product consists of a Nanobody that binds to the Fc-domain of human IgG with high affinity & specificity. Specification Description: Nano-Secondary® alpaca anti-human IgG, Fc-specific recombinant VHH reagent uses a novel class of human IgG-specific antibodies. This secondary antibody product consists of a Nanobody that binds to human IgGs (IgG1, IgG2, IgG3, IgG4) with high affinity & specificity. Due to their small size, Nano-Secondary reagents provide enhanced image resolution. No cross-reactivity against serum proteins from other species apart from slight reactivity to macaque serum, details see below. Applications: IF, FC Host: Alpaca Species Reactivity: Reactivity: human IgG1, IgG2, IgG3, IgG4, Fc-fragment of human IgG, including wt, PGLALA and PALALA mutations. Slight reactivity to macaque serum. No cross-reactivity: human IgA/IgM/IgE, serum proteins from goat, guinea pig, mouse rabbit, rat, sheep. Target/Specificity: Fc-fragment of human IgG (IgG1, IgG2, IgG3, IgG4) Physical State: Liquid Conjugate: CoraLite® Plus 488 Suggested Dilution: Immunofluorescence 1:500 - 1:1000 Flow cytometry: 1µL per 1x10 6 cells Purification Method: Recombinant expression, affinity purification IMAC Type: Secondary Nanobody Class: Recombinant Format: Alpaca single domain antibody, monovalent Cross-reactivity: No cross-reactivity to human IgA/IgM/IgE, and goat, guinea pig, mouse, rabbit, rat, and sheep serum. Shows slight cross-reactivity to macaque serum. Immunogen: Purified human IgG Clonality: Monoclonal Nanobody Clones: CTK0117 (VHH1118) RRID: AB_3075302 Affinity (KD): < 10 pM Excitation / Emission: Excitation max: 493 nm, Emission max: 522 nm Storage Buffer: 10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % Sodium azide Storage Condition: Shipped at ambient temperature. Store at +4°C short term or -20°C long term. Stable for 1 year at -20°C. Size: 10 μL; 100 μL Note: This product is for research use only, not for diagnostic or therapeutic use