Proteintech, srbAF488-1, ChromoTek Nano-Secondary® anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 488 [CTK0101, CTK0102]

Size: 10ul / 100ul Nano-Secondary® anti-human IgG/anti-rabbit IgG, recombinant VHH is an anti-human IgG and anti-rabbit IgG specific secondary antibody. It consists of of a mixture of 2 Nanobodies that bind to human IgG and rabbit IgG with high affinity & specificity. Specification Description: Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH reagent uses a novel class of anti-rabbit IgG and anti-human IgG specific antibodies. This secondary antibody product consists of Nanobodies that bind to rabbit IgG and human IgG with high affinity & specificity. Nano-Secondary reagents enable simultaneous incubation with primary antibodies, thus shortening your experimental. Due to their small size, Nano-Secondary reagents provide enhanced image resolution. No cross-reactivity against serum proteins from other species, details see below. Applications: IF, WB, FC Host: Alpaca Species Reactivity: Rabbit, Human, Macaque No cross-reactivity to mouse, rat, sheep, goat, and guinea pig IgG Target/Specificity: Fc-fragment of human IgG, Fab- and Fc-fragment of rabbit IgG (co-reactivity) Physical State: Liquid Conjugate: Alexa Fluor® 488 Suggested Dilution: Immunofluorescence 1:1,000 Super-resolution microscopy 1:1,000 Western blot 1:1,000 Purification Method: Recombinant expression, affinity purification IMAC Type: Mixture of 2 monoclonal Nanobodies; Secondary Nanobody Class: Recombinant Format: Alpaca single domain antibody, monovalent Cross-reactivity: No cross-reactivity to goat, guinea pig, mouse, rat, and sheep serum Cross-reactivity to macaque serum Immunogen: Purified rabbit IgG Clonality: Biclonal: mixture of 2 monoclonal Nanobodies Clones: CTK0101 (VHH0244): Fc-fragment of human IgG, Fc-fragment of rabbit IgG CTK0102 (VHH0245): Fab-fragment of rabbit IgG Affinity (KD): CTK0101: K D = 0.2 nM, CTK0102: K D = 1.2 nM RRID: AB_2827585 Excitation / Emission: Excitation max: 490 nm, Emission max: 525 nm Storage Buffer: 10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % Sodium azide Storage Condition: Store at -20°C short term or -80°C long term. Aliquot upon delivery. Avoid freeze-thaw cycles. Size: 10 μL; 100 μL Note: This product is for research use only, not for diagnostic or therapeutic use