Qiagen, 180425, QIAseq 1Step Amplicon Lib UDI-D Kit (96)

For 96 reactions: 1-Step Amplicon Enzyme Mix, 4x 1-Step Amplicon Buffer, UDI-D Adapter Plate 96-plex Illumina, Primer Mix Illumina Library Amp, HiFi PCR Master Mix, RNase-Free Water

Features

- One-tube, benchtop library prep from PCR products in just 30 minutes
- Maximal convenience with automatable, 1-reaction, room-temperature setup
- High-quality, artifact-free libraries, ready for use on any Illumina NGS platform
- Compatible with any gene panel or PCR product, only needs 1 ng input DNA
- Single-use adapter plates minimize risk of contamination or handling errors

Performance

Significant time savings – from amplicon to high-quality, NGS-ready library in just 30 minutes

Excellent sequencing metrics

Procedure

The QIAseq 1-Step Amplicon Library Kit offers substantial time savings over standard targeted resequencing library preparations. Utilizing a novel, combined end-repair/ligation reaction, the kit streamlines the entire NGS library preparation process to just 30 minutes. It incorporates a one-tube reaction that saves you time, allowing you to focus on sequencing and data analysis. The entire procedure can be performed at room temperature, enabling automation on instruments lacking a thermo-block. The one-tube protocol eliminates the need for transferring reagents, increasing efficiency and more effectively capturing insert amplicons, while also reducing the risk of contamination or sample mix-up, which can occur with manual processing. The procedure is optionally PCR-free to avoid introducing sequence duplicates or PCR-bias, and generates high-quality libraries optimized for sequencing on any Illumina sequencing instrument from a range of input materials.

Applications

The QIAseq 1-Step Amplicon Library Kit provides an efficient, streamlined, single-reaction solution for amplicon and gene panel sequencing on any Illumina platform.