Qiagen, 220211, QIAGEN OneStep Ahead RT-PCR Kit (50) icon_0368_ls_gen_eco_friendly-s

6 vials for 50 reactions: 1 x 500 µl OneStep Ahead RT-PCR Master Mix, 1 x 50 µl OneStep Ahead RT Mix, 1 x 200 µl Template Tracer, 1 x 50 µl Master Mix Tracer, 1 x 1.9 ml water, 1 x 400 µl Q-Solution

Features

- Unique two-phase hot-start procedure for room-temperature setup
- Dedicated enzyme mix for increased specificity, sensitivity and fidelity
- Faster than ever 1-hour cycling protocol
- Visual pipetting control, resulting in fewer pipetting errors
- Duplex capability, enabling inclusion of internal control or reference gene

Principle

QIAGEN OneStep Ahead RT-PCR: Features
Sensiscript and Omniscript reverse transcriptases: Wide range of RNA amounts (0.1 pg – 2 µg) High sensitivity Heat-mediated activation
QuantiNova HotStarTaq, hot-start high-fidelity Polymerase: Highly specific products Heat-mediated activation Increased fidelity Short cycling time
Master mix with DNA polymerases, Buffers, dNTPs: Proprietary dual-cation buffer system Optimized for duplex PCR
RNase inhibitor: Inhibition of RNases
Q-Solution: Facilitates amplification of GC-rich templates
Optional pipetting control: Inert orange and blue dyes Run-on gel at 50 bp (orange) and 4000 bp (blue)

Procedure

The QIAGEN OneStep Ahead RT-PCR Kit allows fast and easy RT-PCR setup whatever the application – virus detection, molecular diagnostics research or gene expression analysis – just add the RT-enzyme mix, primers and template to the Master Mix in one tube and start the thermal-cycler program (see figure “ OneStep Ahead RT-PCR procedure ”). Reaction setup can conveniently be done at room temperature and the optional master mix and template tracers minimize pipetting errors. The reaction mixture contains all of the reagents required for both reverse transcription and PCR (see table).

Applications

- Virus detection
- Gene expression analysis