Qiagen, 250011, QIAcuity Nanoplate 8.5k 24-well (10)

10 QIAcuity Nanoplate 8.5k 24-well, 11 Nanoplate Seals

Features

- Four nanoplates for different application needs
- Up to 8500 or 26,000 partitions per well
- SBS format

Product Details

The QIAcuity Nanoplates are microfluidic digital PCR plates that enable running 8, 24 or 96 samples with up to 8500 or 26,000 partitions per well. All four nanoplates are designed to run on the QIAcuity digital PCR instruments.

The nanoplates can only be used with the QIAcuity Digital PCR System . Use the dedicated QIAcuity Nanoplate Adapter when performing automated liquid handling and PCR setup in a QIAcuity Nanoplate using the QIAgility . Once done, load the plate onto the QIAcuity Digital PCR System for the dPCR reaction.

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Performance

Type: Frame color
Nanoplate 26K 8-well: Light blue
Nanoplate 26K 24-well: Blue
Nanoplate 8.5K 24-well: White
Nanoplate 8.5K 96-well: Gray

These are specially designed plates used for the digital PCR reactions in the QIAcuity instruments. QIAGEN offers four nanoplate types, all SBS format, but with different specifications for different application needs.

Principle

In just 3 simple steps, you can have the dPCR result you want in under 2 hours: pipette and load, run the experiment, analyze results. The principle of the dPCR reaction in the nanoplates is described here .

Procedure

Just like in qPCR experiments, sample preparation includes the transfer of master mix, probes and primers to a 8-, 24- or 96-well nanoplate, followed by the addition of samples. The system integrates partitioning, thermocycling and imaging into a single fully automated instrument that takes users from the sample to result in under 2 hours. One can perform analysis on the Suite Software, providing the concentration in copies per microliter of your target sequence as well as for quality control such as positive samples or NTC. This analysis can also be extended to remote computers within the same local area network (LAN).

Applications

- Rare mutation detection
- Copy number variation analysis
- Gene expression analysis
- Pathogen detection
- Genotyping
- miRNA research
- Cell and gene therapy
- Residual DNA quantification
- Waste water monitoring

The QIAcuity Nanoplates, in combination with the QIAcuity Digital PCR System and the QIAcuity PCR kits, enable digital PCR applications, including: