Qiagen, 28000-50, AllPrep PowerViral DNA/RNA Kit (50)

For isolating viral or bacterial total nucleic acids from waste water and stool samples

Features

- Optimized to isolate viral or bacterial DNA and RNA from waste water, stool, biosolids and gut material
- Inhibitor Removal Technology efficiently eliminates inhibitory substances
- Highly effective lysis of solid and liquid samples with bead beating protocol

Product Details

The AllPrep PowerViral DNA/RNA Kit is designed for fast and easy purification of viral and bacterial total nucleic acids even from samples high in PCR inhibitors; including waste water, stool, biosolids and gut material. Inhibitor Removal Technology ensures complete removal of inhibitory substances often contained in these materials, such as undigested plant material in the gut or heme compounds from lysed red blood cells that are abundant in stool. The result is DNA and RNA from virus and bacteria that is ready to use in the most demanding downstream applications. Nucleic acids are eluted in RNase-free water and ready to use in PCR, cDNA synthesis, RT-qPCR, RNA-seq, Sanger sequencing or next-generation sequencing. Want to try this solution for the first time? Request a quote for a trial kit .

Performance

- Sample disruption: PowerLyzer 24 Homogenizer
- Sample extraction: AllPrep PowerViral DNA/RNA Kit
- Library preparation: QIAseq 1-step Amplicon Kit (16S rRNA gene sequencing) or QIAseq FX DNA Library Kit (metagenomic shotgun sequencing)
- Analysis: QIAGEN Microbial Genomics Pro Suite (request a demo )

For stool samples or samples with a solid matrix, lysis is achieved using 0.1 mm Glass Bead Tubes in combination with a strong chemical lysis buffer that ensures efficient extraction of tough microorganisms in the bead beating step. For virus extraction from water samples and samples that do not require dispersion, the bead beating step may be skipped and virus extracted through chemical lysis only.

Tools to complete your workflow:

The AllPrep PowerViral DNA/RNA Kit was previously sold by MO BIO as the PowerViral Environmental DNA/RNA Kit.