Qiagen, 339347, miRCURY LNA SYBR Green PCR Kit (4000) icon_0368_ls_gen_eco_friendly-s

For 4000 reactions: 2X miRCURY SYBR Green Master Mix, RNase-Free Water

Features

- Optimized for use with miRCURY LNA miRNA PCR Assays and Panels
- Sensitive and specific detection and quantification of miRNA
- Includes optional ROX dye for optional normalization of fluorescence

Product Details

The miRCURY LNA SYBR Green PCR Kit contains a high-performance PCR master mix specifically designed for use with the miRCURY LNA miRNA PCR System . The miRCURY SYBR Green PCR Kit also features a built-in control for visual identification of correct template addition, as well as Q-Bond, an additive in the PCR buffer that enables short cycling steps without loss of PCR sensitivity and efficiency. The kit has been optimized for use with any real-time cycler. ROX Reference Dye is provided in a separate tube and can be added if your cycler requires ROX as a passive reference dye.

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Performance

Unmatched sensitivity

Universal reverse transcription combined with LNA-enhanced and T m -normalized primers enables accurate and reliable quantification of individual miRNAs from as little as 1 pg total RNA (see figure Accurate quantitation from down to 1 pg total RNA starting material ). With exceptional sensitivity and extremely low background, you can accurately quantify very low levels of miRNA without pre-amplification. This makes the miRCURY LNA miRNA PCR System suitable for all types of samples, especially those with low RNA content, such as serum, plasma and other biofluids.

Principle

High-performance reagents for quantitative, real-time RT-PCR

Applications

- Mature miRNA quantification and profiling
- Mature miRNA and precursor miRNA detection in parallel
- snoRNA RNA detection