Qiagen, 51704, QIAamp DNA Microbiome Kit (50)

For 50 DNA preps: 50 QIAamp UCP Mini Columns, 50 Pathogen Lysis Tubes L, buffers, reagents, Collection Tubes (2 ml)

Features

- Isolation of bacterial DNA from swabs and body fluids
- Effective depletion of host DNA
- Optimized mechanical and chemical cell lysis
- Ultra-clean columns to minimize contamination risk

Product Details

New kit available: The QIAamp DNA Host-Free Microbiome Kit is now available. It isolates microbial DNA from tissue, bodily fluids and swabs and effectively depletes host genomic DNA.

The QIAamp DNA Microbiome Kit is a dedicated solution for the purification and enrichment of bacterial microbiome DNA from swabs and body fluids. Effective depletion of host DNA during the purification process maximizes bacterial DNA coverage in next generation sequencing analysis and allows highly sensitive 16S rDNA-based microbiome analysis and whole metagenome shotgun sequencing studies.

Performance

The high percentage of reads attributed to the host genome in whole metagenome shotgun sequencing experiments makes proper assembly of microbial datasets time consuming and resource intensive, even with sophisticated bioinformatics tools. By removing host DNA in a sample, the QIAamp DNA Microbiome Kit provides samples with enriched bacterial composition for whole metagenome shotgun sequencing. A comparison of reads from whole metagenome shotgun sequencing experiments was performed on DNA isolated from human buccal swabs, where samples were prepared with either the QIAamp DNA Microbiome Kit or solutions from 2 other suppliers. Sequencing of samples prepared with the QIAamp DNA Microbiome Kit resulted in less than 5% human reads, greatly reduced compared to over 90% reads with a kit that does not include host DNA removal and 35% with a kit that does. In a second experiment, samples were spiked with known cultured bacteria. Bacterial DNA were recovered with higher representation compared to a solution without host DNA removal (see figure Effective host DNA removal enhances whole metagenome shotgun sequencing results ).

Improved amplification for 16S rDNA sequencing

16S rDNA sequencing is commonly used to determine relative microbial community composition. The QIAamp DNA Microbiome Kit enables the most efficient amplification of the V4 region of DNA purified from buccal swabs when compared to 3 other purification kits (see figure Efficient amplification and sequencing of 16S rDNA ). Sequencing results of the amplified V4 region from 2 samples purified with the QIAamp DNA Microbiome Kit also exhibit the expected bacterial composition representative of a human oral microbiome.

Applications

- Whole genome sequencing analysis
- Highly-sensitive 16S rDNA-based microbiome analysis
- Metagenomic shotgun sequending studies

The QIAamp DNA Microbiome Kit purifies and enriches bacterial microbiome DNA from mixed samples for use in sensitive downstream applications such as: