Qiagen, 74171, RNeasy 96 QIAcube HT Kit (5)

For 480 preps: RNeasy 96 plates, RNase-free water, buffers

Features

- Simple and reliable automated processing for time and cost savings
- Flexible procedures for isolating total RNA or total RNA, including miRNA
- Protocols for various starting materials and desired size distribution
- High-quality purified RNA ready for use in any downstream application
- Consistent RNA yields from small amounts of starting material

Performance

Cell line: Source
HeLa: Human cervical carcinoma
LMH: Chicken hepatoma
COS-7: Monkey kidney, SV-40 transformed
Huh7: Human hepatoma
Jurkat: Human T-cell leukemia
K-562: Human chronic myelogenous leukemia in blast crisis

Principle

Specification: Description
Number of samples: 24–96 samples (to be processed in increments of 8)
Sample input volume: Up to 5 x 10 5 cells, homogenized in 140 μl Buffer RLT
Elution volume: 110 μl
Duration: 96 samples in approximately 93 or 114* minutes 24 samples in approximately 54 or 73* minutes

Specification: Description
Number of samples: 24–96 samples (to be processed in increments of 8)
Sample input volume: 350 μl aqueous phase from up to 40 mg frozen or 20 mg stabilized tissue homogenized in 750 μl QIAzol Lysis Reagent
Elution volume: 110 μl
Duration: 96 samples in approximately 73 or 93* minutes 24 samples in approximately 34 or 54* minutes

Specification: Description
Number of samples: 24–96 samples (to be processed in increments of 8)
Sample input volume: 350 μl aqueous phase from up to 40 mg frozen or 20 mg stabilized tissue homogenized in 750 μl QIAzol Lysis Reagent
Elution volume: 110 μl
Duration: 96 samples in approximately 78 minutes 24 samples in approximately 38 minutes

The RNeasy 96 QIAcube HT Kit enables automated purification of total RNA or total RNA, including miRNA, from 5 x 10 5 animal or human cells or <40 mg tissue using the QIAcube HT instrument. Six instrument protocols are available, depending on the starting material and desired size distribution of purified RNA. Cell samples can be purified directly using the RNeasy 96 QIAcube HT Kit and the dedicated kit protocol. Tissue samples (<40 mg) and samples for purification of small RNA are processed following sample lysis in QIAzol Lysis Reagent. The procedures yield high-quality RNA that performs well in downstream applications. The RNeasy 96 QIAcube HT Kit combines the selective binding properties of a silica-based membrane with a high-throughput 96-well format, and is designed for fully-automated, simultaneous processing of 24–96 samples on the QIAcube HT instrument.

Applications

- RT-PCR and qRT-PCR
- Differential display
- cDNA synthesis
- Northern, dot, and slot blot analyses
- Primer extension
- Poly A+ RNA selection
- RNase/S1 nuclease protection

The RNeasy 96 QIAcube HT Kit provides efficient, high-throughput RNA sample preparation for research use in fields such as drug screening and basic research. The purified RNA is ready to use in any downstream application, including: