Qiagen, 764114, PAXgene Bone Marrow RNA Tubes (50) icon_0368_ls_gen_eco_friendly-s

50 Bone Marrow Collection Tubes. To be used in conjunction with the PAXgene Bone Marrow RNA Kit

Features

- To be used in conjuction with the PAXgene Bone Marrow RNA Kit
- Immediate stabilization of intracellular RNA upon introducing the sample into the tube
- Rapid purification of RNA from heterogeneous bone marrow samples using the PAXgene Bone Marrow RNA Kit
- RNA is stable for days at room temperature or refrigerated
- Long-term sample storage at –20°C or –70°C

Principle

Copy numbers of individual mRNA species in bone marrow can change significantly during storage or transport at room temperature, making reliable studies of gene expression impossible. PAXgene Bone Marrow RNA Tubes protect RNA molecules from degradation and immediately stabilize the RNA in the sample for storage at room temperature, refrigerated, or frozen.

Procedure

Bone marrow samples are collected using standard medical practices, with commonly used anticoagulants. A 2 ml sample is then transferred into a PAXgene Bone Marrow RNA Tube. The RNA in the sample is immediately stabilized for storage at room temperature, refrigerated, or frozen. The PAXgene Bone Marrow RNA Kit provides an easy and rapid procedure for isolation and purification of intracellular RNA from human whole bone marrow samples. The kit uses silica-membrane technology in a spin-column format for efficient purification of intact, high-quality RNA.

Applications

- cDNA synthesis
- Gene expression arrays
- End-point RT-PCR
- Quantitative RT-PCR, including TaqMan technology

RNA is stabilized in PAXgene Bone Marrow RNA Tubes and can be used in a range of downstream applications, including: