Qiagen, 764133, PAXgene Bone Marrow RNA Kit (30) icon_0368_ls_gen_eco_friendly-s

For 30 RNA preps: 30 PAXgene Spin Columns, 30 PAXgene Shredder Spin Columns, Processing Tubes, RNase-free DNase I, RNase-free reagents and buffers. To be used in conjunction with PAXgene Bone Marrow RNA Tubes

Features

- To be used in conjunction with the PAXgene Bone Marrow RNA Tube
- Rapid purification of RNA with high integrity and purity
- Optimized protocol for heterogeneous bone marrow samples
- Integrated DNase treatment for gDNA removal

Principle

Copy numbers of individual mRNA species in bone marrow can change significantly during storage or transport at room temperature, making reliable studies of gene expression impossible. PAXgene Bone Marrow RNA Tubes protect RNA molecules from degradation and immediately stabilize the RNA in the sample for storage at room temperature, refrigerated, or frozen.

Procedure

Bone marrow samples are collected using standard medical practices, with commonly used anticoagulants. A 2 ml sample is then transferred into a PAXgene Bone Marrow RNA Tube. The RNA in the sample is immediately stabilized for storage at room temperature, refrigerated, or frozen. The PAXgene Bone Marrow RNA Kit provides an easy and rapid procedure for isolation and purification of intracellular RNA from human whole bone marrow samples. The kit uses silica-membrane technology in a spin-column format for efficient purification of intact, high-quality RNA.

Applications

- Real-time RT-PCR analysis of specific transcripts
- Array analysis of complex expression profiles

RNA purified using the PAXgene Bone Marrow RNA Kit is ready for use in a wide range of downstream applications, including