Qiagen, 768536, QIAsymphony PAXgene Blood ccfDNA Kit (192) icon_0368_ls_gen_eco_friendly-s

Reagent cartridges, accessories and proteinase K vials for 192 preps

Features

- Optimized chemistry and input volumes for PAXgene Blood ccfDNA Tubes
- Separate protocol lines for small (STA) and large (LAF) ccfDNA fragments
- Prefilled reagent cartrdiges with bar code for safety and ease of use
- Standardized processing for high reproducibility

Performance

The fully automated QIAsymphony PAXgene Blood ccfDNA Kit procedure generates ccfDNA yields comparable to the manual QIAamp Circulating Nucleic Acid Kit procedure (see figure High performance, comparable to QIAamp Circulating Nucleic Acid Kit ). Since yield is strongly dependent of donor, individual yields may vary. Onboard measures of the QIAsymphony SP minimize cross-contamination.

Procedure

The workflow begins with a centrifugation step that separates plasma from the cellular fraction of the whole blood collected into PAXgene Blood ccfDNA Tubes. The plasma is then carefully transferred into a second tube. A second centrifugation step can be carried out optionally to remove any traces of buffy coat. The pure plasma is then transferred to a third tube which is loaded into the QIAsymphony SP. In the QIAsymphony SP, plasma proteins are digested by proteinase K while ccfDNA binds to the surface of magnetic beads (see figure The QIAsymphony SP processes samples containing magnetic particles ). Depending on the chosen protocol line, predominantly small ccfDNA fragments (STA protocol line) or small and large ccfDNA fragments (LAF protocol line) are isolated. Three wash steps ensure contaminant removal. Finally, ccfDNA is eluted from the magnetic particles and is ready for use in downstream applications (see figure Two protocol lines of the QIAsymphony PAXgene Blood ccfDNA Kit for different research needs ).

Applications

- PCR, digital, multiplex and quantitative real-time PCR
- Pharmacogenomic studies
- SNP genotyping
- Next generation sequencing
- Methylation assays

ccfDNA purified using the QIAsymphony PAXgene Blood ccfDNA Kit and the QIAsymphony SP is ready for use in a wide range of downstream applications, including: