Qiagen, 911060, QIAcuityDx Four

For nanoplate-based digital PCR diagnostic applications.

Features

- For precise and multiplexed quantification results for rare mutation detection, copy number variation (CNV), gene expression studies, gene-editing analysis, and many more
- Integrates a standard dPCR workflow into a walk-away automated platform
- Fully automated processing of QIAcuityDx Nanoplates
- Minimal hands-on time
- Dual mode software: Utility (open) and IVD (closed) modes
- Explore more than 200 dPCR assays on GeneGlobe for use in utility mode for research purposes

Product Details

The QIAcuityDx performs fully automated processing of QIAcuityDx nanoplates , including all necessary steps of nanoplate partitioning, thermocycling and image analysis. Depending on the plate type, up to 96 samples per plate can be analyzed. For diagnostic applications, the QIAcuityDx Nanoplate 26K is available with 24 wells. A total of four nanoplates can be simultaneously processed, with continuous loading possible. The QIAcuityDx Control Software controls all integrated modules, including a robotic gripper for nanoplate handling, a partitioning module, a PCR thermocycler and a fluorescence imaging module.

Performance

Operating modes: IVD (closed) Utility (open)
Plates processed per run: 4
Plate loading style: Continuous loading
Detection channels (multiplexing): 5
Time to result of first plate: 120 minutes
Each subsequent plate: 80 minutes
Throughput: 8 plates –192 samples Dx mode (Dx 24 well, 26,000 partition plate)* 8 plates – 768 samples Utility Mode (Research Use: 96 well, 8,000 partition plate)*
Dimensions: Width 60 cm (23.6 in.) / Height 58 cm (22.8 in.) / Depth 65 cm (25.6 in.)

The QIAcuityDx Digital PCR System makes absolute quantification accessible for all laboratories. The walk-away automation integrates and streamlines the entire dPCR workflow of partitioning, thermocycling and imaging into a single instrument with minimal hands-on time. No change in plate handling is required when coming from qPCR, assuring fast assay setup and quick results in approximately two hours for the first plate.

QIAcuityDx Instruments – features and specifications

*Based upon an 8-hour working day

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Principle

The QIAcuityDx is designed as a walk-away instrument that integrates and automates all plate processing steps. Only the nanoplate preparation must be done manually before starting the run. If this preparation is done and the experiment is set up, the nanoplate must be placed in a free plate slot of the instrument tray. By reading the barcode of the plate, the instrument links the nanoplate to the experimental parameters previously defined in the software. After initiating the run, all further steps are performed, fully automated, by the instrument.

Procedure

Just like in a qPCR workflow, sample preparation includes the transfer of master mix, probes and primers to any QIAcuity plate type* followed by the addition of samples. The system integrates partitioning, thermocycling and imaging into a single, fully automated instrument that achieves sample to result in under 2 hours. One can perform analysis on the Software Suite, providing the concentration in copies per microliter of your target sequence as well as for quality control such as positive samples or NTC. This analysis can also be extended to remote computers within the same local area network (LAN).

* An IVD variant of the 24-well, 26,000 partition nanoplate will be available for use with QIAcuityDx, in either Utility Mode or IVD Mode. Utility Mode, intended for translational research or for laboratories to develop their own developed tests workflow, will allow the use of QIAcuity research use plate variants.

Applications

- Rare mutation detection and quantitation
- Copy number variation analysis
- Gene expression analysis
- Pathogen detection and quantitation
- Genotyping

The QIAcuityDx may be used for a wide range of applications, including, but not limited to, oncology and infectious disease testing. From a biological risk perspective, the QIAcuityDx is a ‘closed’ system once a top-seal is applied to a nanoplate, thus significantly reducing the risk of instrument contamination and potential user infection.

The QIAcuityDx instrument, together with the QIAcuityDx Nanoplates and the QIAcuityDx PCR master mixes and assays, enable clinical dPCR applications , including:

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