Qiagen, 941558, BioSprint 96 DNA Plant Kit (1536)

For 1536 preps: Large 96-Rod Covers, 96-Well Microplates MP, S Blocks, MagAttract Suspension G, buffers and reagents

Features

- High-throughput isolation of DNA
- Maximal flexibility to accommodate challenging sample material
- Complete removal of contamination and inhibitors
- No organic extraction or alcohol precipitation

Product Details

BioSprint DNA Plant Kits provide all reagents and plasticware necessary for automated purification of total DNA from plant tissue using proven magnetic-particle technology on the BioSprint 15 or BioSprint 96.

Performance

Sample type: Amount
Arabidopsis, fresh leaves: 50–120 mg
Cotton, fresh leaves: 50 mg
Maize, fresh leaves: 50 mg
Pine, fresh needles: 100–190 mg
Rose, fresh new leaves: 100–140 mg
Rose, fresh old leaves: 100–140 mg
Soybean, fresh leaves: 50 mg
Spruce, fresh needles: 130–160 mg
Wheat, fresh leaves: 50 mg
Maize, lyophilized leaves: 5–10 mg
Rapeseed, lyophilized leaves: 5–10 mg
Sunflower, lyophilized leaves: 5–10 mg
Maize, dried seed powder: 20–55 mg
Apple: 1–6 seeds
French bean: 1 bean
Grape: 3 seeds
Linen: 1–10 seeds
Lupine: 2–7 seeds
Orange: 1 seed
Rapeseed: 1–4 seeds
Soybean: 1 seed
Swedish turnip: 5–50 seeds
Tomato: 15–30 seeds
Turnip radish: 4–18 seeds
Wheat: 3–4 seeds

DNA can be successfully purified from a variety of plant species and material (see tables “DNA purified from a wide range of plant species using the BioSprint 96”).

DNA purified from a wide range of plant species using the BioSprint 96

Purified DNA is up to 40 kb in size (see figure " Reproducible purification of high-quality DNA "). The DNA is ready to use in a range of downstream applications, including PCR and real-time PCR, genotyping and microsatellite analysis (see figures " High PCR performance of DNA purified using the BioSprint 15 DNA Plant Kit ” and " High PCR performance of purified DNA ”).

Principle

BioSprint DNA Plant Kits are used with the BioSprint 15 and BioSprint 96. The kits use magnetic-particle technology for rapid DNA purification from plant tissue. BioSprint instruments control an array of magnetic rods that can attract or release magnetic particles and transfer them from tube to tube or well to well. The sequential transfer of magnetic particles allows a rapid purification procedure to be performed, from the initial binding of DNA to the particles, through to washing of the particles and elution of pure DNA (see figure " Efficient processing of magnetic particles "). Since the instruments transfer magnetic particles instead of liquids, they use minimal amounts of reagents, enabling cost-efficient sample preparation.

Procedure

BioSprint DNA Plant Kits enable purification of total DNA from a wide variety of plant species, including difficult plant material. Fresh, frozen or lyophilized starting material is mechanically disrupted to give a fine powder using a homogenizer such as the TissueLyser LT or TissueLyser II. The powder is resuspended in lysis buffer, thoroughly mixed and then sedimented by a short centrifugation step. Cleared plant lysates are transferred to a 5-well strip or S-Block and loaded into the instrument, which then performs all steps until pure DNA is obtained (see flowchart " BioSprint DNA Plant procedure "). The kits provide all the necessary reagents and plasticware for sample preparation, and are available in different sizes, allowing cost-efficient use of consumables.

Applications

- PCR
- Real-time PCR
- Genotyping
- Microsatellite analysis

BioSprint DNA Plant Kits purify DNA for use in a range of downstream applications, including: