Revvity, 6016731, ATPlite 1step Luminescence Assay System, 100 mL ATP Assay Kit

Overview
ATP is a marker for cell viability due to its presence in all metabolically active cells. ATP concentration declines rapidly when cells undergo necrosis or apoptosis, and so monitoring ATP is a good indicator of cytotoxic, cytostatic and proliferation effects.
Our ATPlite 1step ATP luminescence assays use patented technologies to measure cell proliferation and cytotoxicity in mammalian cells based on the production of light caused by the reaction of ATP with added luciferase and D-luciferin.
Features and benefits:

Simple and reproducible: only one reagent addition step, no separation steps
Suitable for both 96- and 384-well microplates
Linear correlation between cell number and luminescent signal: up to 50,000 cells per well for 96-well microplates and 12,500 cells per well for 384-well microplates
Designed for continuous process systems: luminescence should be measured between 0 and 30 minutes after reagent addition and plate shaking
Homogeneous: no cell harvesting or centrifugation required
High sensitivity: able to detect down on one cell per well
Reduced Phenol Red dependency
High light-output: assay can be used with less sensitive luminescence readers such as multi-label readers
Fast: no luminescence signal stabilization time required

An alternative format of this assay is also available. The ATPlite assay has separate cell lysis and luminescent signal generation steps. This allows for a more stable signal, with half-life of at least 4 hours. Application-Cell Viability
Assay Points-1000
Automation Compatible-Yes
Brand-ATPlite 1step
Cellular or Signaling Pathway-Autophagy Cell Death Cell Growth Cytotoxicity Necrosis
Detection Modality-Luminescence
Format-Microplates
Protocol-1-step
Signal Halflife-0.5 hour
Shipping Conditions-Shipped Ambient
Unit Size-100 mL