Revvity, 6055508, PhenoPlate 96-well, black, optically clear flat-bottom, poly-D-lysine-coated, lids, case of 160

Overview
These PhenoPlate 96-well microplates (formerly named CellCarrier Ultra microplates) for high content imaging applications are poly-D-lysine (PDL)-coated and are available in black in a 96-well format. The microplates benefit from a film bottom made of cyclic olefin, a plastic that has glass-like optical properties, providing superior image quality and also good cell adherence. They have an ultra-low plate bottom (0.2mm) for better well access when using water immersion and high NA objectives, and a new design, low profile polystyrene lid for reduced evaporation.
Poly-lysine is a synthetic positively-charged polymer, existing as two enantiomers: Poly-D-lysine (PDL) and Poly-L-lysine (PLL). Adherence of certain cell types to poly-lysine-coated surfaces is based on the electrostatic interaction of the poly-D-lysine polycation with the negative charges of the cell membrane. Use of poly-lysine coatings on plate surfaces can help mediate the negative charges of the cell membrane and the negative charge of the surface. Both PDL and PLL are commonly used however PDL is not degraded by cellular proteases and is therefore often the preferred choice. As Poly-lysine is a synthetic protein, it does not influence the signaling pathways of the cells and is completely free of any animal contaminants. Almost all cell types will adhere to Poly-lysine coated plate bottoms.
Packaging notes and storage:

Cases of 40 are packaged as 2 sleeves of 20 plates, lids included
Cases of 160 are packaged as 8 sleeves of 20 plates, lids included
Storage Temperature: 4°C

Well plate dimensions

Well format: 96-well

Description

Specification

Number of rows

8

Number of columns

12

Well volume

425 µL

Recommended working volume

Up to 300 µL

Height (mm)

14.35

Length (mm)

127.76

Width (mm)

85.48

Well diameter top (mm)

6.84

Well diameter bottom (mm)

6.4

Well depth (mm)

12.7

A1 to top offset (mm)

11.24

A1 to side offset (mm)

14.38

Well-to-well spacing (mm)

9 Color-Black/Clear Bottom
Plate Format-96 wells
Well Shape-Flat-bottom
Well Volume-425 µL
Working Volume-Up to 300 µL
Application-High Content Imaging Microscopy
Automation Compatible-Yes
Barcoding Compatibility-Yes
Brand-PhenoPlate
Detection Modality-Fluorescence
Format-Microplates
Lids Included?-Yes
Material-Cyclic Olefin
Packaging-8 sleeves of 20 plates
Quantity-160 plates
Shipping Conditions-Shipped Ambient
Sterility-Aseptic
Storage Conditions-4°C
Surface Treatment-Poly-D lysine coated